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M9480525.TXT
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1994-08-20
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Document 0525
DOCN M9480525
TI Quantitative analysis of viral burden in tissues from adults and
children with symptomatic human immunodeficiency virus type 1 infection
assessed by polymerase chain reaction.
DT 9410
AU Sei S; Kleiner DE; Kopp JB; Chandra R; Klotman PE; Yarchoan R; Pizzo PA;
Mitsuya H; Medicine Branch, National Cancer Institute, NIH, Bethesda,
MD; 20892.
SO J Infect Dis. 1994 Aug;170(2):325-33. Unique Identifier : AIDSLINE
MED/94308597
AB The amount of human immunodeficiency virus type 1 (HIV-1) in various
tissues was investigated by polymerase chain reaction (PCR) in 16
patients with end-stage HIV-1 infection and 7 patients with symptomatic
but less advanced disease. During postmortem study of the 16 end-stage
patients, HIV-1 DNA was found most often in lymph nodes and the spleen
(both 100%), lung (93.8%), and colon (87.5%). Biopsied lymph nodes from
the 7 symptomatic patients contained substantially higher copy numbers
of HIV-1 RNA and DNA than did peripheral blood mononuclear cells (PBMC).
Plasma viral RNA levels correlated significantly with the amount of
HIV-1 RNA in PBMC (r2 = .86, P = .0025) but not with the level of viral
RNA in lymph nodes in patients with symptomatic HIV-1 infection. These
data suggest that although lymph nodes represent the main site for HIV-1
infection and replication, the level of circulating viral burden may not
be solely determined by the magnitude of active HIV-1 replication in
lymph nodes.
DE Acquired Immunodeficiency Syndrome/*MICROBIOLOGY/MORTALITY Adult
Central Nervous System/MICROBIOLOGY Child Child, Preschool
Colon/MICROBIOLOGY DNA, Viral/*ANALYSIS Female Gene Expression
Regulation, Viral Human HIV-1/*GROWTH &
DEVELOPMENT/GENETICS/PHYSIOLOGY Infant Leukocytes,
Mononuclear/MICROBIOLOGY Lung/MICROBIOLOGY Lymph Nodes/*MICROBIOLOGY
Lymphoid Tissue/MICROBIOLOGY Male Middle Age Polymerase Chain
Reaction RNA, Messenger/BIOSYNTHESIS RNA, Viral/ANALYSIS/BLOOD
Spleen/*MICROBIOLOGY Thymus Gland/MICROBIOLOGY Viremia/MICROBIOLOGY
Virus Replication JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).